Oligo(dT)18 primers are designed for efficient and selective reverse transcription of eukaryotic mRNA by annealing to the poly(A) tail of mature transcripts. These primers are critical in ensuring high specificity, enabling the generation of full-length cDNA while minimizing interference from rRNA and tRNA. Unlike random hexamers, which provide broad priming, Oligo(dT)18 primers ensure that only polyadenylated RNA is reverse-transcribed, making them ideal for high-purity mRNA studies.

Supplied at a 50 µM concentration, Oligo(dT)18 primers are optimized for use with various reverse transcriptase’s, including those requiring thermostable conditions. Their length (18 nucleotides) ensures stable hybridization to poly(A) tails while reducing premature termination, allowing the synthesis of full-length transcripts. The well-defined priming strategy enhances reproducibility, particularly in applications such as RT-qPCR, RNA sequencing, and cDNA cloning.

The high purity of Oligo(dT)18 primers minimizes primer-dimer formation and non-specific annealing, critical for maintaining reaction efficiency. Additionally, these primers are compatible with one-step and two-step RT protocols, providing flexibility in experimental design. Their performance is validated across multiple RNA sources, ensuring reliability in gene expression analysis and transcriptome studies.

By offering high specificity, purity, and consistency, Oligo(dT)18 primers provide researchers with a robust tool for accurate and efficient cDNA synthesis, ultimately improving the fidelity of downstream molecular biology applications.

Single Pack Contents

OligodT18 (50µM): 50µl X 5 vials