| Product Name | Nicotinamide Adenine Dinucleotide (NAD) |
| Species | General |
| Test Method | Competitive Inhibition |
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length | 2h |
Specificity
This assay has high sensitivity and excellent specificity for detection of Nicotinamide Adenine Dinucleotide (NAD). No significant cross-reactivity or interference between Nicotinamide Adenine Dinucleotide (NAD) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of Nicotinamide Adenine Dinucleotide (NAD) and the recovery rates were calculated by comparing the measured value to the expected amount of Nicotinamide Adenine Dinucleotide (NAD) in samples.
| Matrix | Recovery Range(%) | Average Recovery(%) |
| serum(n=5) | 96-105 | 101 |
| EDTA plasma(n=5) | 87-102 | 96 |
| heparin plasma(n=5) | 85-97 | 93 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nicotinamide Adenine Dinucleotide (NAD) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nicotinamide Adenine Dinucleotide (NAD) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-102% | 79-90% | 98-105% | 91-99% |
| EDTA plasma(n=5) | 89-104% | 92-101% | 94-102% | 89-96% |
| heparin plasma(n=5) | 87-96% | 82-95% | 89-103% | 91-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
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