|
Amount |
100μg/100μl |
|
Form of Antibody |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
|
Immunogen |
The antiserum was produced against synthesized non-phosphopeptide derived from human NF-κB p65 around the phosphorylation site of serine 529 |
|
Swiss-Prot No |
Q04206 |
|
Altername |
NF-KB p65, NFKB3, Nuclear factor NF-kappa-B p65 subunit, RELA, TF65, Transcription factor p65, p65 |
|
Purification |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
|
Specificity/Sensitivity |
NF-κB p65 (Ab-529) antibody detects endogenous levels of total NF-κB p65 protein |
|
Applications |
WB: 1:500~1:1000 IHC: 1:50~1:100, ELISA: 1:10000 |
|
References |
Xu C, et al (2005) Oncogene:24(28): 4486-95.
McNulty SE, et al. (2004) Pigment Cell Res Apr; 17(2): 173-80.
Madrid LV,et al. (2001) J Biol Chem: 276(22): 18934-40.
Wang D, et al. (2000) J Biol Chem : 275(42): 32592-7 |
|
Peptide - + |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-κB p65 (Ab-529) antibody (#B7162). |
