|
NEFA (Non-Esterified Fatty Acid) ELISA Kit |
|
Sensitivity |
0.01 μmol/mL |
|
Range |
0.0313μmol/mL-2μmol/mL |
|
Alternative Names |
NEFA |
|
Detection Method |
Competitive |
|
Standard |
2μmol/mL |
|
Sample Type |
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids, Sample volume: 50μL |
|
Reaction time |
1.5H |
|
Research Area |
Metabolic pathway; Signal Transduction; Cancer, Nutrition metabolism;Hormone metabolism; Signal transduction;Metabolic pathway; Enzyme & Kinase; Signal transduction;Metabolic pathway; Nutrition metabolism;Hormone metabolism;Cardiovascular，Signaling transduction，Neuroscience，Cancer，Metabolism; Enzyme & Kinase;Infection immunity;Endocrinology;Hormone metabolism; Metabolic pathway; Signal Transduction; Cance |
|
Principle |
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with NEFA. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to NEFA. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NEFA in the samples is then determined by comparing the OD of the samples to the standard curve. |
|
Validated Image |
|
