BCIP/NBT (5-Bromo-4-chloro-3-indolyl phosphate/Nitro blue tetrazolium) Substrate Solution is a high-performance chromogenic substrate for alkaline phosphatase (AP) used in molecular biology, immunology, and biochemical assays. This ready-to-use reagent produces a strong purple precipitate upon reaction with alkaline phosphatase, enabling clear and reliable visual detection of target molecules.

Ideal for Western blotting, ELISA, and in situ hybridization, BCIP/NBT Substrate Solution delivers high sensitivity and excellent contrast in AP-based detection systems. In this formulation, BCIP acts as the substrate, while NBT functions as the color-developing reagent, ensuring stable, long-lasting results. Its optimized composition provides sharp visualization and accurate analysis for both research and diagnostic applications.