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Na⁺k⁺-ATPase Activity Assay Kit |
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Sensitivity |
0.11 μmol Pi/mL/hour |
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Range |
0.42-4.99 μmol Pi/mL |
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Alternative Names |
Na+K+-ATPase |
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Sample Type |
Serum,Plasma,Animal tissue |
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Detection Method |
Colorimetric method |
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Detection Instrument |
Microplate reader (640-680 nm) |
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Research Area |
Oxidative Stress |
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Other Reagents Required |
Normal saline (0.9% Na |
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Storage |
2-8°C for 12 months |
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Precision |
inter-assay CV: 2.2% | intra-assay CV: 2.2% |
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Sample Volume |
200 μL |
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Assay Time |
35 min |
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Principle |
Na+K+ -ATPase decomposes ATP to produce ADP and Phosphorus, and calculates the activity of Na+K+ -ATPase by measuring the content of phosphorus. |
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Na+/K+ ATPase Activity Colorimetric Microplate Assay Kit |
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Description |
Detection and Quantification of Na+/K+ ATPase Activity. |
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Alternative Names |
EC 3.6.3.9; Sodium/potassium-exchanging ATPase; Na(+)/K(+)-ATPase; Na(+)/K(+)-exchanging ATPase; Na,K-activated ATPase; Sodium pump; Sodium/potassium-transporting ATPase |
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CAS Number |
9000-83-3 |
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Detection Method |
Colorimetric |
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Detection Range |
10 μmol/L - 1000 μmol/L |
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Sample Types |
Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media, Other biological fluids |
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Platform |
Microplate reader 650 nm |
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Storage |
store at 4°C for 6 months. |
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Principle |
Na+/K+ ATPase is a crucial transmembrane ion pump that utilizes energy from ATP hydrolysis to actively transport three sodium ions out of the cell and two potassium ions into the cell. This electrochemical gradient not only maintains cellular osmotic balance and resting potential but also underlies fundamental physiological processes such as neuronal excitation, muscle contraction, and renal reabsorption. Na+/K+ATPase Activity Colorimetric Microplate Assay Kit provides a convenient tool for sensitive detection of Na+/K+ATPase activity in a variety of samples. In this assay, ATPase hydrolyzes ATP releasing ADP and a free phosphate ion. The dye reagent forms a color with released phosphate ion, which is measured on a plate reader 650 nm. |
