RNase Inhibitor is a specific ribonuclease (RNase) inhibitor found in human placenta. The principle of its specific inactivation of RNase is to bind non-covalently with RNase to form a complex, causing RNase to become inactive.

This product is a recombinant mouse RNase inhibitor expressed and purified in a soluble form in E. coli, capable of broadly inhibiting various types of RNases (RNase A, B, C). Tested with RT-PCR and RT-qPCR, this product is compatible with Hifair® III Reverse Transcriptase (Cat#11111ES) and various DNA Polymerases. Compared to human RNase inhibitors, this product lacks two cysteine residues that are highly sensitive to oxidation in human proteins, thus possessing greater antioxidant activity. Additionally, it is more suitable for experiments with high sensitivity to DTT, such as qPCR.

This product can be used in experiments such as first-strand cDNA synthesis, separation of polyribosomes, in vitro transcription, and in vitro cell-free translation systems.

Exonuclease, Incision Enzyme Residue

Detection of MRI nuclease (in a 20 μl reaction system, 200 U of MRI and 0.5 μg of λDNA-HindⅢ digest were added, incubated at 37°C for 4 hours) and nicking enzyme residual activity (in a 20 μl reaction system, 200 U of this enzyme and 0.5 μg of plasmid DNA were added, incubated at 37°C for 4 hours) shows no residual activity. Note: N represents the negative control; 1, 2, 3 represent three different batches.

RNase Enzyme Residue

20 μl reaction system added 200 U MRI and 0.5 μg 293T RNA, incubated at 37℃ for 4 hours, agarose gel electrophoresis RNA band did not change, there was no RNase residue.