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MT (Melatonin) ELISA kit |
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Sensitivity |
9.38 pg/mL |
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Range |
15.63-1000pg/mL |
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Uniprot ID |
N/A |
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Detection Method |
Competitive |
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Standard |
1000pg/mL |
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Sample Volume |
50μL |
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Sample Type |
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids |
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Reaction time |
1.5H |
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Research Area |
Metabolic pathway; Signal Transduction; Cancer, Nutrition metabolism;Hormone metabolism; Signal transduction;Metabolic pathway; Enzyme & Kinase; Signal transduction;Metabolic pathway; Nutrition metabolism;Hormone metabolism;Cardiovascular，Signaling transduction，Neuroscience，Cancer，Metabolism; Enzyme & Kinase;Infection immunity;Endocrinology;Hormone metabolism; Metabolic pathway; Signal Transduction; Cancer |
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Principle |
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with MT. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to MT. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MT in the samples is then determined by comparing the OD of the samples to the standard curve. |
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Validated Image |
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