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MRS Broth (Lactobacillus MRS Broth)

MRS Broth (Lactobacillus MRS Broth)

Catalouge No BSDCM-1185
HSN NO. 38210000
Size 100 GM | 500 GM
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SKU: BSDCM-1185 Category:
Physical Parameters
Description A fine, free flowing, hygroscopic powder
Color Light tan colored powder
Solubility Soluble in Distilled / de-ionized water at 50˚C
Color Light amber colored opalescent
pH 6.5 ±0.2 at 25 ˚C (55.15 g/ l)
Contents
Ingredients Gram/Litre
Proteose Peptone 10.00
Meat Extract 10.00
Yeast Extract 5.00
Dextrose 20.00
Sorbitan Monooleate 1.00
Ammonium Citrate 2.00
Sodium Acetate 5.00
Magnesium Sulfate 0.10
Manganese Sulfate 0.05
Potassium Phosphate Dibasic 2.00
pH at 25˚C 6.5 ±0.2
For isolation and cultivation of Lactobacillus species.
Dissolve 55.15 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically. Incubate in 5% CO2 atmosphere at 33-37°C for 24-72 hrs.