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MRS Agar (Lactobacillus MRS Agar)

MRS Agar (Lactobacillus MRS Agar)

Catalouge No BSDCM-1180
HSN NO. 38210000
Size 100 GM | 500 GM
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SKU: BSDCM-1180 Category:
Physical Parameters
Description A fine, free flowing, hygroscopic powder
Color Light tan colored powder
Solubility Soluble in Distilled / de-ionized water at 50˚C
Color Light amber colored opalescent
pH 6.5 ±0.2 at 25 ˚C (67.50 g/ l)
Solidification 40 ˚C
Color Light amber colored opalescent gel
Contents
Ingredients Gram/Litre
Proteose Peptone 10.00
Meat Extract 10.00
Yeast Extract 5.00
Dextrose 20.00
Sorbitan Monooleate 1.00
Ammonium Citrate 2.00
Sodium Acetate 5.00
Magnesium Sulfate 0.10
Manganese Sulfate 0.05
Potassium Phosphate Dibasic 2.00
Agar 12.00
pH at 25˚C 6.5 ±0.2

For the isolation and enumeration of lactic acid bacteria from meat and meat products.

Dissolve 67.50 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample aseptically. Incubate in 5% CO2 atmosphere at 33-37°C for 24-72 hrs.