Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SP in the samples is then determined by comparing the OD of the samples to the standard curve.
| Catalogue No. | BSELK1511 |
| Size | 48T/96T/5*96T/10*96T |
| Product Category | ELISA (Quantitative) |
| Reactivity | Mouse |
| Sample | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Type: | Sandwich |
| Assay Length | 3.5h |
| Sensitivity | 4.63 pg/mL |
| Range | 15.63-1000 pg/mL |
| Storage/Storage | The unopened kit should be stored at 2 – 8°C. After opening, please store refer to the manual. |
| Precautions | The product listed herein is for research use only and not intended for diagnosis field. The international literature is also constantly updated, it is best to confirm with the salesman or email us before ordering. The product listed herein all was produced by our laboratory. If you have any other requirements, please email us, to get you wanted product information.info@bio-string.com |
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