| Mouse SIL-6R (Soluble Interleukin 6 Receptor) Elisa Kit | |
| Sensitivity | 0.122 ng/mL |
| Range | 0.32-20 ng/mL |
| Alternative Names | IL-6R; CD126; gp80; IL6R; IL-6R-1; IL-6R-Alpha; IL6RA; IL-6 receptor subunit alpha; Membrane glycoprotein 80; IL6R; Interleukin 6 Receptor |
| Detection Method | Sandwich |
| Standard | 20 ng/mL |
| Sample Type | serum, plasma, tissue homogenates and other biological fluids |
| Assay Length | 3.5h |
| Research Area | Signal Transduction, CD & Adhesion Molecule, Infection Immunity |
| Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse sIL-6R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse sIL-6R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse sIL-6R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse sIL-6R in the samples is then determined by comparing the OD of the samples to the standard curve. |
Mouse SIL-6R (Soluble Interleukin 6 Receptor) Elisa Kit
| Catalogue No | BSEK-317M |
| Species | Mouse |
| Size | 96 T | 48 T |
| Price | POR |
| Concentration (ng/mL) | OD | Corrected OD |
| 20.00 | 2.131 | 2.044 |
| 10.00 | 1.563 | 1.476 |
| 5.00 | 1.193 | 1.106 |
| 2.50 | 0.895 | 0.808 |
| 1.25 | 0.513 | 0.426 |
| 0.63 | 0.361 | 0.274 |
| 0.32 | 0.216 | 0.129 |
| 0.00 | 0.087 | 0.000 |
| 20.00 | 2.131 | 2.044 |
- Intra-assay Precision (Precision within an assay): CV%<8%
- Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
- Inter-assay Precision (Precision between assays): CV%<10%
- Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrices listed below were spiked with certain levels of recombinant sIL-6R and the recovery rates were calculated by comparing the measured value to the expected amount of sIL-6R in samples.
| Matrix | Recovery Range | Average |
| serum(n=5) | 92-107% | 99% |
| EDTA plasma(n=5) | 87-99% | 93% |
| Heparin plasma (n=5) | 78-92% | 85% |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sIL-6R and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 95-104% | 82-96% | 92-105% | 83-92% |
| EDTA plasma(n=5) | 87-96% | 85-98% | 89-102% | 85-98% |
| Heparin plasma (n=5) | 80-101% | 87-96% | 96-105% | 95-103% |