|
Product Name |
Mouse sIL-6R(SolubleInterleukin 6 Receptor) ELISA Kit |
|
Reactivity |
Mouse |
|
Alternative Names |
IL-6R; CD126; gp80; IL6R; IL-6R-1; IL-6R-Alpha; IL6RA; IL-6 receptor subunit alpha; Membrane glycoprotein 80; IL6R; Interleukin 6 Receptor |
|
Assay Type |
Sandwich |
|
Sensitivity |
0.122 ng/mL |
|
Standard |
20 ng/mL |
|
Detection Range |
0.32-20 ng/mL |
|
Sample Type |
serum, plasma, tissue homogenates and other biological fluids |
|
Assay Length |
3.5h |
|
Research Area |
Signal Transduction, CD & Adhesion Molecule, Infection Immunity |
Standard Curve
|
Concentration (ng/mL) |
OD |
Corrected OD |
|
20.00 |
2.131 |
2.044 |
|
10.00 |
1.563 |
1.476 |
|
5.00 |
1.193 |
1.106 |
|
2.50 |
0.895 |
0.808 |
|
1.25 |
0.513 |
0.426 |
|
0.63 |
0.361 |
0.274 |
|
0.32 |
0.216 |
0.129 |
|
0.00 |
0.087 |
0.000 |
|
20.00 |
2.131 |
2.044 |
Precision
- Intra-assay Precision (Precision within an assay): CV%<8%
- Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
- Inter-assay Precision (Precision between assays): CV%<10%
- Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain levels of recombinant sIL-6R and the recovery rates were calculated by comparing the measured value to the expected amount of sIL-6R in samples.
|
Matrix |
Recovery Range |
Average |
|
serum(n=5) |
92-107% |
99% |
|
EDTA plasma(n=5) |
87-99% |
93% |
|
Heparin plasma (n=5) |
78-92% |
85% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sIL-6R and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Matrix |
1:2 |
1:4 |
1:8 |
1:16 |
|
serum(n=5) |
95-104% |
82-96% |
92-105% |
83-92% |
|
EDTA plasma(n=5) |
87-96% |
85-98% |
89-102% |
85-98% |
|
Heparin plasma (n=5) |
80-101% |
87-96% |
96-105% |
95-103% |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse sIL-6R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse sIL-6R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse sIL-6R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse sIL-6R in the samples is then determined by comparing the OD of the samples to the standard curve.
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