Product Name |
Mouse IL-6(Interleukin 6) ELISA Kit |
Catalogue No. |
1210ME |
Synonyms |
IL-6 ELISA Kit, Interleukin 6 ELISA Kit, IL6 ELISA Kit, BSF2 ELISA Kit, HGF ELISA Kit, HSF ELISA Kit, IFNB2 ELISA Kit, CDF ELISA Kit |
Detection Method |
Sandwich ELISA, Double Antibody |
Application |
IL-6 ELISA Kit allows for the in vitro quantitative determination of IL-6 concentrations in serum, plasma, tissue homogenates and other biological fluids. |
Size |
96T |
Range |
15.625-1000pg/ml |
Sensitivity |
< 9.375pg/ml |
Species |
Mouse |
Standard Curve |
|
Storage |
2-8 ℃ for 6 months |
Recovery |
Matrices listed below were spiked with certain level of IL-6 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-6 in samples.
Matrix |
Recovery range(%) |
Average(%) |
serum(n=5) |
88-105 |
97 |
EDTA plasma(n=5) |
86-102 |
92 |
heparin plasma(n=5) |
87-103 |
98 |
|
Linearity |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample |
1:2 |
1:4 |
1:8 |
serum(n=5) |
89-102% |
93-104% |
85-104% |
EDTA plasma(n=5) |
82-97% |
86-98% |
85-99% |
heparin plasma(n=5) |
84-98% |
80-91% |
85-98% |
|
CV(%) |
Intra-Assay: CV<8%
Inter-Assay: CV<10% |
Note |
For Research Use Only |
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