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Mouse IL-6(Interleukin 6) ELISA Kit |
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Sensitivity |
0.2 pg/mL |
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Range |
1.56-100pg/mL |
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Uniprot ID |
P08505 |
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Detection method |
Sandwich |
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Standard |
100pg/mL |
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Sample type |
Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL |
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Reaction time |
3.5H |
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Research Area |
Cardiovascular biology |
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Alternative Names |
IL-6;IL 6;IL6;Interleukin-6;Interleukin 6;Interleukin6;B-cell differentiation factor, B-cell stimulatory factor 2, BSF2, BSF-2, CDF, CTL differentiation factor , HSF, hybridoma growth factor, IFNB2, IFN-beta-2, IL6, IL-6, Interferon beta-2, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6, MGI-2A;Hybridoma growth factor;Interferon beta-2;IFNB 2;IFNB-2;IFN-B2 |
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Principle |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-6. You can calculate the concentration of Mouse IL-6 in the samples by comparing the OD of the samples to the standard curve. |
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Mouse IL-6(Interleukin 6) ELISA Kit |
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Sensitivity |
9.375pg/ml |
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Range |
15.625-1000pg/ml |
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Detection method |
Sandwich ELISA, Double Antibody |
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Application |
IL-6 ELISA Kit allows for the in vitro quantitative determination of IL-6 concentrations in serum, plasma, tissue homogenates and other biological fluids. |
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Sample type |
Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL |
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Alternative Names |
IL-6 ELISA Kit, Interleukin 6 ELISA Kit, IL6 ELISA Kit, BSF2 ELISA Kit, HGF ELISA Kit, HSF ELISA Kit, IFNB2 ELISA Kit, CDF ELISA Kit |
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Mouse IL-6(Interleukin 6) ELISA Kit |
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Sensitivity |
1.6 ng/L |
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Range |
8 ng/L -150 ng/L |
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Detection method |
Sandwich-ELISA |
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Storage/Stability |
2-8℃. |
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Sample type |
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. |
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Principle |
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to IL-6. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for IL-6 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IL-6 and HRP conjugated IL-6 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IL-6. You can calculate the concentration of IL-6 in the samples by comparing the OD of the samples to the standard curve. |
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Mouse IL-6(Interleukin 6) ELISA Kit |
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Sensitivity |
21.8 pg/ml |
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Range |
31.25pg/ml-2000pg/ml |
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Detection method |
Sandwich-ELISA |
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Storage/Stability |
2-8℃. |
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Sample type |
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. |
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Principle |
This ELISA kit uses Sandwich-ELISA as the method. The Micro Elisa strip plate provided in this kit has been pre-coated with an antibody specific to IL-6. Standards or samples are added to the appropriate Micro Elisa strip plate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for IL-6 is added to each Micro Elisa strip plate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IL-6 and HRP conjugated IL-6 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IL-6. You can calculate the concentration of IL-6 in the samples by comparing the OD of the samples to the standard curve. |
