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Mouse IL-10(Interleukin-10) ELISA Kit |
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Sensitivity |
9.375pg/ml |
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Range |
15.625-1000pg/ml |
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Alternative Name |
Interleukin-10 ELISA Kit, IL-10 ELISA Kit, Cytokine synthesis inhibitory factor ELISA Kit, CSIF ELISA Kit, IL10 ELISA Kit |
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Sample Type |
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
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Detection Method |
Sandwich ELISA, Double Antibody |
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Wavelength |
OD450 |
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Reaction Duration |
4 hours |
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Specificity |
Specifically binds with IL-10 , no obvious cross reaction with other analogues. |
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Storage |
2-8°C(Sealed), Don't cryopreserve. |
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Principle |
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL-10 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-10 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-10 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value. |
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Validated Image |
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