| Mouse G-CSF(Granulocyte Colony Stimulating Factor) ELISA Kit - Details | |
| Sensitivity | 9.375pg/ml |
| Range | 15.625-1000pg/ml |
| Alternative Names | Granulocyte colony-stimulating factor ELISA Kit, G-CSF ELISA Kit, Pluripoietin ELISA Kit, CSF3 ELISA Kit, C17orf33 ELISA Kit, GCSF ELISA Kit |
| Detection method | Sandwich ELISA, Double Antibody |
| Sample Type | Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
| Reaction Time | 4 hours |
| Detection Wavelength | OD450 |
| UniProt ID | P09920 |
| Specificity | Specifically binds with G-CSF , no obvious cross reaction with other analogues. |
| Storage | 2-8°C (Sealed), Don't cryopreserve. |
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti G-CSF antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with G-CSF bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of G-CSF in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.