|
Product Details |
|
Product Name |
Mouse BNP(Brain Natriuretic Peptide) ELISA Kit |
|
Uniprot ID |
P40753 |
|
Detection method |
Competitive |
|
Standard |
1000pg/mL |
|
Sample type |
Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL |
|
Reaction time |
1.5H |
|
Research Area |
Endocrinology |
|
Alternative Names |
NPPB, GC-B, B-Type Natriuretic Peptide, Natriuretic Peptide Precursor B |
|
Test Principle |
|
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse BNP. During the reaction, Mouse BNP in the sample or standard competes with a fixed amount of Mouse BNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse BNP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse BNP in tested samples can be calculated by comparing the OD of the samples to the standard curve. |
Reviews
There are no reviews yet.