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Product Details |
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Product Name |
Mouse ALB(Albumin) ELISA Kit |
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Uniprot ID |
N/A |
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Assay Type |
Competitive Inhibition |
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Standard |
100 µg/mL |
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Sample type |
serum, plasma and other biological fluids |
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Assay Length |
2h |
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Research Area |
Tumor immunity; Infection immunity; Hematology; Hepatology; Nutrition metabolism |
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Alternative Names |
Serum albumin |
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Test Principle |
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This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse ALB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ALB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ALB in the samples is then determined by comparing the OD of the samples to the standard curve. |
