Dulbecco’s Modified Eagle Medium-Low Glucose (DMEM) Powder

Dulbecco's Modified Eagle Medium-Low Glucose (DMEM) Powder- With 1gm Glucose per liter, L-Glutamine and Sodium pyruvate and Without Sodium bicarbonate
Product Name Modified Eagle Medium-Low Glucose (DMEM) Powder
Catalogue No CMP8525P-1L
Quantity 10.11 g of powder per litre
Storage 2-8° C
Shipping condition Ambient
Self-life 36 months from the date of manufacturing
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Dulbecco’s Modified Eagle Medium (DMEM) is a widely used cell culture medium derived from Eagle’s medium. It is an enriched version of Basal Medium Eagle (BME), containing four times the concentration of amino acids and vitamins. DMEM also includes glycine, serine, and ferric nitrate. The original formulation, developed for culturing embryonic mouse cells, contains 1000 mg/L of glucose.

Composition

Ingredients - INORGANIC SALTS
Calcium chloride dihydrate 265.00
Ferric nitrate nonahydrate 0.100
Magnesium sulphate anhydrous 97.67
Potassium chloride 400.00
Sodium chloride 6400.00
Sodium dihydrogen phosphate monohydrate 109.00
AMINO ACIDS
Glycine 30.00
L-Alanine 84.00
L-Arginine hydrochloride 84.0
L-Cystine dihydrochloride 62.57
L-Glutamine 584.00
L-Histidine hydrochloride-H2O 42.00
L-Isoleucine 105.00
L-Leucine 105.00
L-Lysine hydrochloride 146.00
L-Methionine 30.00
L-Phenylalanine 66.00
L-Serine 42.00
L-Threonine 95.00
L-Tryptophan 16.00
L-Tyrosine disodium salt 103.79
L-Valine 94.00
VITAMINS
Choline chloride 4.00
D-Calcium pantothenate 4.00
Folic Acid 4.00
Niacinamide 4.00
Pyridoxal hydrochloride 4.00
Thiamine hydrochloride 4.00
Riboflavin 0.400
i-Inositol 7.20
OTHERS
D-Glucose 1000.00
Phenol red sodium salt 15.9
Sodium pyruvate 110.00
  • Suspend the pouch content in 900 ml of ultra-pure water or water for injection grade suitable for tissue culture with constant stirring until the powder is completely dissolved.
  • Weigh 3.7 g of sodium bicarbonate powder and add to 1 litre of medium and stir until dissolved.
  • Adjust the pH to ~0.25 (pH 7.0 to 7.4) below the desired pH using 1N HCl or 1N NaOH, since the pH tends to rise during filtration process.
  • Make up the volume to 1000ml with water.
  • Sterilize filter through a sterile membrane filter with a porosity of 0.22μm, using positive pressure to avoid the loss of carbon dioxide.
  • Aseptically add sterile supplements as required and aliquot sterile medium into sterile cell culture grade bottles.
  • Store liquid medium at 2-8°C till further use.

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