| Application | MEM is used for the culture of adherent cells, and can also be used for other types of cell culture after the formulation is revised. |
| Description | MEM including NEAA (non-essential amino acids) medium, is made on the basis of MEM added seven NEAA, including L-alanine, L-glutamic acid, L-asparagine, L-proline, aspartic acid, L-glycine, serine and can reduce the cell culture cells themselves when the side effects of synthetic non-essential amino acids, effectively promote the metabolism of cell proliferation. And This product contains amino acids, vitamins, inorganic salts and other components needed for multi-type cell culture, without protein or growth factor. Serum or serum free additives should be added according to the type of cell. This product is only used for lab research, not for diagnosis and treatment. |
| Note | It is recommended to monitoring the pH of the medium. If needed, pH can be adjusted by using sterile 1N HCl or 1N NaOH. |
| Directions for Use | 1.Suspend the pouch content (13.3 g) in 900 ml of ultra-pure water or water for injection grade suitable for tissue culture with constant stirring until the powder is completely dissolved.
2.Weigh 2.2 g of sodium bicarbonate powder and add to 1 litre of medium and stir until dissolved. 3.Adjust the pH to ~0.25 (pH 7.0 to 7.4) below the desired pH using 1N HCl or 1N NaOH, since the pH tends to rise during filtration process. 4.Make up the volume to 1000 ml with water. 5.Sterilize filter through a sterile membrane filter with a porosity of 0.22µm, using positive pressure to avoid the loss of carbon dioxide. 6.Aseptically add sterile supplements as required and aliquot sterile medium into sterile cell culture grade bottles. 7.Store liquid medium at 2-8°C till further use. |
Minimum Essential Medium Eagle (MEM)
| With Earle’s salts, non-essential amino acids (NEAA), L-Glutamine, Sodium Pyruvate, 15mM HEPES and without Sodium bicarbonate | |
| Catalog No. | CM8532P |
| Quantity | 13.3 g of powder per litre |
| Pack Size | 1L / 10L |
| Storage | 2-8° C |
| Quantity | 10g of powder per litre |
| Self-life | 36 months from the date of manufacturing |
| Shipping condition | Ambient |
|
Composition: Ingredients |
mg/L |
|
INORGANIC SALTS Calcium chloride dihydrate |
265.00 |
|
Magnesium sulphate anhydrous |
97.72 |
|
Potassium chloride |
400.00 |
|
Sodium bicarbonate |
2200.00 |
|
Sodium chloride |
6800.00 |
|
Sodium dihydrogen phosphate anhydrous |
122.00 |
|
AMINO ACIDS Glycine |
7.50 |
|
L-Alanine |
8.90 |
|
L-Arginine hydrochloride |
126.00 |
|
L-Asparagine monohydrate |
15.00 |
|
L-Aspartic acid |
13.30 |
|
L-Cystine dihydrochloride |
31.30 |
|
L-Glutamic acid |
14.70 |
|
L-Glutamine |
292.00 |
|
L-Histidine hydrochloride monohydrate |
42.00 |
|
L-Isoleucine |
52.00 |
|
L-Leucine |
52.00 |
|
L-Lysine hydrochloride |
72.50 |
|
L-Methionine |
15.00 |
|
L-Phenylalanine |
32.00 |
|
L-Proline |
11.50 |
|
L-Serine |
10.50 |
|
L-Threonine |
48.00 |
|
L-Tryptophan |
10.00 |
|
L-Tyrosine disodium salt dihydrate |
51.900 |
|
L-Valine |
46.000 |
|
VITAMINS |
|
|
Choline chloride |
1.00 |
|
D-Ca-Pantothenate |
1.00 |
|
Folic acid |
1.00 |
|
Nicotinamide |
1.00 |
|
Pyridoxal hydrochloride |
1.00 |
|
Riboflavin |
0.10 |
|
Thiamine hydrochloride |
1.00 |
|
i-Inositol |
2.00 |
|
OTHERS |
|
|
D-Glucose |
1000.00 |
|
Phenol red sodium salt |
11.00 |
|
HEPES buffer |
3575.00 |
|
Sodium Pyruvate |
110.00 |
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