Product Introduction
Connective tissue, in a narrow sense, refers to the three types of fibers it contains: collagen fibers, reticular fibers, and elastic fibers. Among them, collagen fibers are the most widely distributed and abundant. Masson trichrome staining, also known as Masson staining, is the most classical method for connective tissue staining and is an authoritative and traditional technique for collagen fiber visualization.
The so-called “trichrome” staining typically refers to staining the nucleus and selectively displaying collagen and muscle fibers. The staining principle is related to the size of anionic dye molecules and tissue permeability: smaller molecules penetrate dense, low-permeability tissues more easily, while larger molecules only enter loosely structured, high-permeability tissues. Since light green or aniline blue have large molecular weights, after Masson staining, muscle fibers appear red, while collagen fibers appear green (light green) or blue (aniline blue). This method is mainly used to distinguish collagen fibers from muscle fibers.
Product Components
| Components | 7x 50mL | 7x 100mL | |
|
Reagent (A): Weigert Iron |
A1: Weigert Solution A | 25 mL | 50 mL |
| A2: Weigert Solution B | 25 mL | 50 mL | |
| Mix A1 and A1 in equal parts before use. Do not prepare in advance. | |||
| Reagent (B): Acid Ethanol Differentiation Solution | 50 mL | 100 mL | |
| Reagent (C): Masson Blue Solution | 50 mL | 100 mL | |
| Reagent (D): Ponceau Fuchsin Staining Solution | 50 mL | 100 mL | |
| Reagent (E): Weak Acid Solution | 50 mL | 100 mL | |
| Reagent (F): Phosphomolybdic Acid Solution | 50 mL | 100 mL | |
| Reagent (G): Aniline Blue Staining Solution | 50 mL | 100 mL | |
Masson Trichrome Staining Solution Features
- Stable staining
- Short differentiation time
- Clear and bright colors
- Wide application range
- suitable for paraffin and frozen sections
- Stained sections can be stored for a long time without fading.
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