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Lactate Dehydrogenase (LDH) Activity Assay Kit |
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Sensitivity |
6U/L |
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Range |
6-500U/L |
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Alternative Names |
LDH |
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Detection Method |
Colorimetric method |
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Sample Types |
Serum, Plasma, Hydrothorax, Tissue, Cell |
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Platform |
Microplate reader (440-460 nm) |
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Research Area |
Liver And Renal Function, Glycolysis And Lipid Metabolism, Energy Metabolism |
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Precision |
inter-assay CV: 2.4% | intra-assay CV: 1.8% |
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Sample Volume |
40 μL |
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Assay Time |
45 min |
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Other Reagents Required |
Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
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Storage |
2-8°C for 12 months |
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Principle |
Using coenzyme I as a hydrogen carrier, LDH catalyze lactic acid to produce pyruvate. Pyruvate reacted with 2, 4-dinitrophenylhydrazine to form pyruvate dinitrophenylhydrazone, which was red-brown in alkaline solution, and the color depth was proportional to pyruvate concentration. The activity of LDH could be calculated by measuring OD value. |
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Validated Image |
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