| Conjugation | Unconjugated |
| Modification | Unmodified |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle. |
| Purification | Affinity purification |
| Gene Name | MKI67 |
| Gene ID | 4288 |
| SwissProt ID | P46013 |
| Dilution Ratio | WB 1:1000-1:10000, IHC-P/IF-P 1:200-1:1000, ICC/IF 1:200-1:1000, FCM 1:1000-1:10000 |
| Molecular Weight | 359kDa |
Background
Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly(PubMed:27362226). Associates with the surface of the mitotic chromosome, the peri chromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly . Associates with the surface of the mitotic chromosome, the peri chromosomal layer, and covers a substantial fraction of the chromosome surface . Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility . Binds DNA, with a preference for supercoiled DNA and AT-rich DNA . Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization . It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Reviews
There are no reviews yet.