| KEAP1 Monoclonal Antibody (1F10B6) | |
| Isotype | Mouse |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1F10B6 |
| Immunogen | Purified recombinant fragment of human KEAP1 (amino acids: 380-624) expressed in E. Coli. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Conc. not determined |
| Storage buffer | ascites |
| Contains | 0.03% sodium azide |
| Storage conditions | Store at 4°C short term |
| Applications | Tested Dilution |
| Western Blot (WB) | 1:500-1:2,000 |
| Immunohistochemistry (Frozen) (IHC (F)) | - |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:200-1:400 |
| ELISA (ELISA) | 1:10,000 |
| Proximity Ligation Assay (PLA) (PLA) | - |
KEAP1 Monoclonal Antibody (1F10B6)
| Catalogue No | BSAB-266P |
| Host | Mouse |
| Reactivity | Human, Mouse |
| Applications | WB, IHC (F), ICC/IF, FC |
| Size | 100 µL |
| Price | POR |
MA5-17106 targets KEAP1 in indirect ELISA, FACS, ICC, IF and WB applications and shows reactivity with Human and Mouse samples.
The MA5-17106 immunogen is purified recombinant fragment of human KEAP1 (amino acids: 380-624) expressed in E. Coli.
MA5-17106 detects KEAP1 which has a predicted molecular weight of approximately 69.7kDa.
This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.
Immunofluorescence analysis of HeLa cells using KEAP1 monoclonal antibody
Western blot analysis of KEAP1 using KEAP1 monoclonal antibody
Western blot analysis of KEAP1 using a KEAP1 monoclonal antibody
Flow cytometric analysis of HepG2 cells using KEAP1 monoclonal antibody