| KD-Validated Bcl-2 Recombinant Rabbit Monoclonal Antibody | |
| Description | KD-Validated Antibody |
| Conjugation | Unconjugated |
| Modification | Unmodified |
| Isotype | Rabbit IgG |
| Clonality | Rabbit mAb |
| Form | Liquid |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Buffer | Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide. |
| Purification | Affinity Purification |
| Gene Name | BCL2 |
| Gene ID | 596 |
| SwissProt ID | P10415 |
| Alternative Names | BCL2 Apoptosis Regulator; PPP1R50; Bcl-2; Protein Phosphatase 1, Regulatory Subunit 50; Apoptosis Regulator Bcl-2; B-Cell CLL/Lymphoma 2; BCL2, Apoptosis Regulator |
| Buffer | Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide. |
| Molecular Weight | Calculated MW: 26.3kDa |
| Dilution Ratio | WB 1:1,000-1:5,000; FC 1:200-1:2,000; IHC-P 1:100-1:200 |
| Research Area | Cell Biology, Signal Transduction, Cancer, Kits/ Lysates/ Other, Metabolism |
| Background | This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. |
| Validated Image |
Western blotting analysis using Bcl-2 antibody (KVAb00250). Bcl-2 expression in wild type (WT) and BCL2 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Bcl-2 antibody (KVAb00250, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (APS0635, 1:10,000) respectively 
Validation of Bcl-2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Bcl-2 antibody (KVAb00250, 1:2,000) and analyzed using BD flow cytometer |
KD-Validated Bcl-2 Recombinant Rabbit Monoclonal Antibody
| Catalogue No | BSAB-316P |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB,FCM,IHC-P |
| Size | 20μL | 50μL | 100μL |
| Price | POR |