Description
| Product Name | INSIG1 Antibody |
| Catalogue No. | DF12412 |
| Size | 100ul |
| Form | liquid |
| Purification | The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific). |
| Source: | Rabbit |
| Clonality | Polyclonal |
| Specificity: | INSIG1 Antibody detects endogenous levels of total INSIG1. |
| Conjugate: | Unconjugated. |
| Storage | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt. |
IMAGES
| Western blot analysis of extracts from Sp2/0, using INSIG1 Ab. Lane 1 was treated with the blocking peptide. |  |
| DF12412 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary Ab was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary Ab. |  |
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