The Kit integrates phenol/guanidine-based lysis and silica membrane purification of total RNA. Mag Zol Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of samples and inhibit RNases. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable extracting up to 200μg total purified RNA from less than 100mg animal, plant, fungal samples, bacteria, cells and other samples.
| Features | Specifications |
| Main Functions | Isolation total RNA (include miRNA) from 100mg lipid tissue, tissue, cell, plant, body fluids using columns and MagZol reagent |
| Applications | RT-PCR, qRT-PCR, Northern Hybridization, Second Generation Sequencing |
| Purification Method | Mini Spin Column |
| Purification Technology | Silica technology, acid phenol / guanidine extraction technology (MagZol pretreatment technology) |
| Process Method | Manual (centrifugation or vacuum) |
| Sample Type | Animal tissue, muscle fiber containing tissue, adipose tissue, cultured cells, lymphocytes, simple plants and other biological samples |
| Sample Amount |
Cultured Cells:5 x 106 |
| Animal Tissue: 1-60mg (spleen≤ 10mg) | |
| Plant Leaves: 50-150 mg | |
| Yield | 2-200μg |
| Elution Volume | ≥50μl |
| Time Per Run | ≤30 minutes（1-24 samples） |
| Liquid carrying volume per column | 800µl |
| Binding Yield of Column | 100µg |
Kit Contents
| Contents | BSR-413002 | BSR-413003 |
| Purification Times | 50 Preps | 250 Preps |
| HyPure RNA Mini Columns | 50 | 250 |
| 2ml Collection Tubes | 50 | 250 |
| MagZol Reagent | 60 ml | 270 ml |
| Buffer RW1 | 50 ml | 200 ml |
| Buffer RW2* | 20 ml | 50 ml |
| RNase Free Water | 10 ml | 30 ml |
Storage and Stability
MagZol Reagent should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
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