Introduction
This kit combines bead grinding and column purification techniques and is suitable for rapid extraction of high-purity total DNA from ≤200mg stool samples. The purification column selects high-performance ultrafine glass fiber filter membrane as the material, which efficiently and specifically adsorbs DNA. This kit adopts silica gel column purification technology and a unique salt mediated adsorption method, which can efficiently remove RNA and inhibitory factors. The entire process does not require phenol chloroform and can efficiently adsorb humic acid and other inhibitory factors in the solution. Purified DNA can be directly used for experiments such as PCR, Southern hybridization, enzyme digestion, and second-generation sequencing.
Details – Kit Contents
| Contents | BSD314102F | BSD314103F |
| Purification Times | 50 Preps | 250 Preps |
| HyPure DNA Mini Columns II | 50 | 250 |
| 2ml Collection Tubes | 50 | 250 |
| 2ml Bead Tubes | 50 | 250 |
| Proteinase K | 24 mg | 120 mg |
| Protease Dissolve Buffer | 1.8 ml | 10 ml |
| Buffer STL | 40 ml | 180 ml |
| Buffer PCI | 20 ml | 90 ml |
| Buffer SL | 5 ml | 25 ml |
| Buffer GWP | 70 ml | 2 x 170 ml |
| Buffer GW2* | 20 ml | 2 x 50 ml |
| Elution Buffer | 15 ml | 60 ml |
Storage and Stability
Proteinase K and Buffer PCI should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
Reviews
There are no reviews yet.