HyPure Soil DNA Purification Kit provides a fast and easy method for purification of total DNA from environmental samples containing a high humic acid content including difficult soil types such as compost, sediment, manure and other common soil types, for reliable applications in PCR and other downstream applications. The HyPurA® DNA purification system combines the reversible nucleic acid binding properties of Hi Elute Miniprep Spin Column (Capped) with an efficient buffer system, which eliminates PCR inhibiting compounds such as humic acid from soil samples.

HyPure Soil DNA Purification Kit

HyPurA® Soil DNA Purification Kit simplifies isolation of DNA from soil using spin column technique. The kit is optimized to process 250-500 mg of soil sample. Soil samples possess typically many compounds that can inhibit downstream enzymatic reactions. The HyPurA® Soil DNA Purification Kit effectively removes these inhibitory compounds during the extraction process. The Inhibitor Removal Solution (IRSH), provided in the kit, is effective in removing PCR inhibitors from even the most difficult soil types. The soil sample is lysed and homogenized in a bead beating step. The total genomic DNA obtained is bound to silica membrane in a spin column format, washed and eluted from the membrane. The purified DNA can directly be used for PCR analysis and other downstream applications.

Concentration, yield and purity of DNA

Spectrophotometric analysis and agarose gel electrophoresis will reveal the concentration and the purity of the genomic DNA. Use Elution Buffer (ET) or Molecular Biology Grade Water to dilute samples and to calibrate the spectrophotometer, measure the absorbance at 260 nm, 280 nm, and 320 nm using a quartz microcuvette. Absorbance readings at 260 nm should fall between 0.1 and 1.0. The 320 nm absorbance is used to correct for background absorbance. An absorbance of 1.0 at 260 nm corresponds to approximately 50 µg/ml of DNA. The A260–A320 A280–A320 ratio should be 1.6–1.9. Purity is determined by calculating the ratio of absorbance at 260 nm to absorbance at 280 nm. DNA purified by HyPurA® Soil DNA Purification Kit is free of protein and other contaminants that can inhibit PCR or other enzymatic reactions. Concentration of DNA sample (µg/ml) = 50 x A260 x dilution factor.