HyPure FFPE RNA Plus Kit

CAT NO PRODUCT NAME SIZE PRICE
BSR-414402 HyPure FFPE RNA Kit 50 preps $189.00
BSR-414403 HyPure FFPE RNA Plus Kit 250 preps $845.00
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SKU: BSR-414402 Categories: ,

HyPure FFPE RNA Kit supplies a simple and rapid RNA extraction for Formalin-fixed, paraffin-embedded (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 30 minutes (not including digestion time). RNA can be directly used for downstream applications such as RT-PCR, Northern blot, vitro translation and other experiments.

Specifications

Features Specifications
Main Functions Isolation total RNA from FFPE tissue and section samples (with DNase)
Applications RT-PCR, quantitative RT-PCR, Northern hybridization, Poly A purification, nucleic acid protection and in vitro translation
Purification Method Mini Spin Column
Purification Technology Silica Technology, DNase
Process Method Manual (Centrifugation or Vacuum)
Sample Type FFPE Tissue Sample
Sample Amount 6mg
Yield 20μg
Elution Volume ≥10μl
Time Per Run ≤60 minutes
Liquid carrying volume per column 800µl
Binding Yield of Column 100µg

Kit Contents

Contents BSR-414302 BSR-414303
Purification Times 50 Preps 250 Preps
HyPure RNA Micro Columns 50 250
2ml Collection Tubes 50 250
Buffer DPS 60 ml 250 ml
Buffer FRL 15 ml 60 ml
Buffer RLC 15 ml 60 ml
Buffer RWC* 10 ml 50 ml
Buffer RW2* 20 ml 2 x 50 ml
DNase I 600 µl 5 x 600 µl
DNase Booster Buffer 1.5 ml 6 ml
Protease Dissolve Buffer 1.8 ml 10 ml
Proteinase K 24 mg 120 mg
RNase Free Water 10 ml 20 ml

This product is based on silica column purification. Remove paraffin by Buffer DPS. Sample lysis with proteinase K digestion requires only 15 minutes. After lysis, samples are incubated at 80ºC for 15 minutes. Transfer to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, RNA was finally eluted with low-salt buffer.

Advantages

  • High quality - high purity total RNA can be directly used in various sensitive downstream applications
  • Fast - several samples can be extracted in 60 minutes by column method
  • Safe - no phenol chloroform extraction required
  • Sensitive - RNA can be recovered at the level of PG
  • Efficient DNA removal – unique method to effectively remove genomic DNA

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