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Hyp(Hydroxyproline) ELISA Kit - Details |
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Sensitivity |
9.38ng/mL |
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Range |
15.63-1000ng/mL |
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Uniprot ID |
N/A |
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Alternative Names |
(2S,4R)-4-Hydroxyproline; L-Hydroxyproline(C5H9O3N) |
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Detection Method |
Competitive |
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Standard |
1000ng/mL |
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Sample type |
Serum, Plasma, Other biological fluids,Sample volume: 50ÎĽL |
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Reaction time |
1.5H |
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Research Area |
N/A |
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Principle |
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Hyp. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Hyp. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hyp in the samples is then determined by comparing the OD of the samples to the standard curve. |
