Medium supplement composed of albumin, insulin, transferrin, cytokines, a cholesterol source, other defined organic and inorganic compounds and fetal calf serum (final concentration: 0.5%). HFCS contains human proteins. The raw material from which the human proteins were isolated, has been tested for the presence of Hepatitis B surface Antigen (HBsAg) and HIV-1/2 antibodies and found to be negative (according to current quality control procedures).
| Synonyms | HFCS |
| sterility | 0.2 μm filtered |
| technique(s) | cell culture | hybridoma: suitable |
| impurities | Mycoplasma, tested 10 EU/mL Endotoxin (LAL) |
| solubility | water: miscible |
| storage temp. | −20°C |
Application
Hybridoma Fusion and Cloning Supplement (HFCS) is used as a supplement to the normal culture medium to support the growth of B-cell hybridomas after fusion and during cloning. HFCS replaces feeder cells. It is also used to optimize the growth of hybridomas after the thawing of cells stored in liquid nitrogen.
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