| Generic Name |
Human Tumor Necrosis Factor Alpha (TNF-α)ELISA Kit |
| Purpose |
Our Human Tumor Necrosis Factor Alpha (TNF-α) ELISA kit is to assay Tumor Necrosis Factor Alpha (TNF-α)levels in Human serum, plasma, culture media or any biological fluid. |
| Principle |
This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to TNF-α Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for TNF-α is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain TNF-α and HRP conjugated TNF-α antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of TNF-α . You can calculate the concentration of TNF-α in the samples by comparing the OD of the samples to the standard curve. |
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