• Get In Touch (814) 262-7331
  • 633, Napoleon Street Johnstown, Pennsylvania PA,15901

Catalogue No. D004Hu
Size 96T
Alternative Names (Cusotm kit)Human Tumor Necrosis Factor Alpha (TNF-α)ELISA Kit
Reactivity Human
Assay range 15.6pg/ml – 1000 pg/ml
Sensitivity 5.5 pg/ml

Description

Generic Name Human Tumor Necrosis Factor Alpha (TNF-α)ELISA Kit
Purpose Our Human Tumor Necrosis Factor Alpha (TNF-α) ELISA kit is to assay Tumor Necrosis Factor Alpha (TNF-α)levels in Human serum, plasma, culture media or any biological fluid.
Principle This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to TNF-α Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for TNF-α is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain TNF-α and HRP conjugated TNF-α  antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of TNF-α . You can calculate the concentration of TNF-α  in the samples by comparing the OD of the samples to the standard curve.

Reviews

There are no reviews yet.

Be the first to review “Human Tumor Necrosis Factor Alpha (TNF-α)ELISA Kit”

Your email address will not be published. Required fields are marked *