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Human Total SOD (Superoxide Dismutase) ELISA Kit |
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Sensitivity |
0.469ng/ml |
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Range |
0.781-50ng/ml (10000IU/mg) |
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Alternative Name |
ALS ELISA Kit, ALS1 ELISA Kit, homodimer ELISA Kit, IPOA ELISA Kit, SOD ELISA Kit, SOD1 ELISA Kit, Superoxide dismutase [Cu Zn] ELISA Kit |
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Sample Type |
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
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Detection Method |
Sandwich ELISA, Double Antibody |
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Wavelength |
OD450 |
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Reaction Duration |
4 hours |
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Specificity |
Specifically binds with SOD , no obvious cross reaction with other analogues. |
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Storage |
2-8°C(Sealed), Don't cryopreserve. |
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Principle |
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti SOD antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with SOD bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of SOD in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value |
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Validated Image |
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