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Human TLR4(Toll Like Receptor 4) ELISA Kit |
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Sensitivity |
0.1ng/ml |
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Range |
1.56ng/mL–50ng/ml |
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Sample Type |
use with serum, plasma, culture media, or any biological fluids |
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Detection Method |
Quantitative Detection |
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Storage |
2-8℃ |
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Principle |
The quantitative detection of Human TLR4 antigen is based on the ELISA (Enzyme-Linked Immunosorbent Assay) technique. The microplate wells are pre-coated with an antibody specific to Human TLR4. After incubation, the corresponding antigen in the sample or standards will bind to the immobilized antibody. After washing the microplate wells to remove all unbound sample material, horseradish peroxidase (HRP) labeled antibody conjugate is added, which binds to the antigen, forming antibody-antigen-enzyme-labeled antibody complex upon incubation. In a second wash step, all unbound material is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate, which gives a blue color reaction in wells containing the complex. A stop solution containing acid terminates the reaction, producing a yellow end-point coloration proportional to the amount of specific antigen present in the sample. Absorbance is measured at 450 nm using an ELISA microwell plate reader. Analysis is completed by comparing the OD values of samples to the standard curve. |
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Human TLR4(Toll Like Receptor 4) ELISA Kit |
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Sensitivity |
0.5ng/mL |
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Range |
0.78-50ng/mL |
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Sample Type |
use with serum, plasma, culture media, or any biological fluids |
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Detection Method |
Sandwich-ELISA |
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Storage |
2-8℃ |
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Principle |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TLR4. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TLR4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TLR4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human TLR4. You can calculate the concentration of Human TLR4 in the samples by comparing the OD of the samples to the standard curve. |
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Human TLR4(Toll Like Receptor 4) ELISA Kit |
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Sensitivity |
0.115 ng/mL |
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Range |
0.32-20 ng/mL |
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Alternative Names |
CD284; TOLL; HToll |
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Assay Type |
Sandwich |
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Standard |
20 ng/mL |
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Sample Type |
tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
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Assay Length |
3.5h |
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Research Area |
Signal transduction, Enzyme & Kinase, Tumor immunity, Infection immunity, Pulmonology |
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Uniprot ID |
O00206 |
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Principal |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TLR4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TLR4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TLR4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TLR4 in the samples is then determined by comparing the OD of the samples to the standard curve. |
