| Catalogue No. | E4353hu |
| Size | 96T |
| Alternative Names | Tight junction protein 1;Tight junction protein ZO-1;TJP 1;TJP1;Zona occludens protein 1;Zonula occludens protein 1 |
| Reactivity | Human |
| Assay range | 20-6000ng/L |
| Sensitivity | 9.46ng/L |
| Assay type | Sandwich |
| Sample type | Serum, plasma, cell culture supernates |
| Assay time | 1h 30m |
| Background | TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed: 7798316, PubMed: 9792688). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells (PubMed: 21240187). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (PubMed: 20930113). With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity). |
| Research area | Tags & Cell Markers |
| Target protein | TJP1 |
| Standard Curve |  |
| Note | For Research Use Only |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E4353hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 3200ng/L | 1.997 | 1.967 | 1.926 |
| 1.938 | |||
| 1600ng/L | 1.04 | 0.983 | 0.942 |
| 0.926 | |||
| 800ng/L | 0.603 | 0.551 | 0.51 |
| 0.498 | |||
| 400ng/L | 0.243 | 0.193 | 0.152 |
| 0.142 | |||
| 200ng/L | 0.083 | 0.099 | 0.058 |
| 0.116 | |||
| 0ng/L | 0.044 | 0.041 | 0 |
| 0.038 |
Precision
We measured random samples of E4353hu within the same batch/lot to ensure the consistency of the kits’ performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 670.5 | 34.49 | 5.1 |
| Intra-Assay | 2 | 18 | 2254 | 52.88 | 2.3 |
| Intra-Assay | 3 | 18 | 1603.1 | 75.31 | 4.7 |
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