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Human REN(Renin) ELISA Kit |
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Sensitivity |
18.75pg/ml |
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Range |
31.25-2000pg/ml |
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Detection Method |
Sandwich ELISA, Double Antibody |
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Sample Type |
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
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Alternative Names |
Renin ELISA Kit, Angiotensinogenase ELISA Kit, REN ELISA Kit |
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Detection Wavelength |
OD450 |
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Assay length |
4 hours |
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Specificity |
Specifically binds with REN , no obvious cross reaction with other analogues. |
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Uniprot ID |
P00797 |
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Principle |
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti REN antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with REN bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of REN in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value. |
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Validated Image |
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