| Catalogue No. |
0904HE |
| Size |
96T |
| Synonyms |
PDE5A ELISA Kit, cGMP-specific 3′ ELISA Kit, 5′-cyclic phosphodiesterase ELISA Kit, cGMP-binding cGMP-specific phosphodiesterase ELISA Kit, CGB-PDE ELISA Kit, PDE5 ELISA Kit |
| Species |
Human |
| Range |
0.156-10ng/ml |
| Sensitivity |
< 0.094ng/ml |
| Detection Method |
Sandwich ELISA, Double Antibody |
| Application |
PDE5A ELISA Kit allows for the in vitro quantitative determination of PDE5A concentrations in serum, plasma, tissue homogenates and other biological fluids. |
| Standard Curve |
 |
| Storage |
2-8 ℃ for 6 months |
| Recovery |
Matrices listed below were spiked with certain level of PDE5A and the recovery rates were calculated by comparing the measured value to the expected amount of PDE5A in samples.
| Matrix |
Recovery range(%) |
Average(%) |
| serum(n=5) |
87-99 |
95 |
| EDTA plasma(n=5) |
88-99 |
94 |
| heparin plasma(n=5) |
85-105 |
95 |
|
| Linearity |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDE5A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample |
1:2 |
1:4 |
1:8 |
| serum(n=5) |
91-103% |
85-100% |
96-102% |
| EDTA plasma(n=5) |
84-94% |
83-91% |
84-96% |
| heparin plasma(n=5) |
83-94% |
82-95% |
91-100% |
|
| CV(%) |
Intra-Assay: CV<8%
Inter-Assay: CV<10% |
| Note |
For Research Use Only |
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