|
Human OxLDL (Oxidized Low Density Lipoprotein) ELISA Kit |
|
Sensitivity |
18.75pg/ml |
|
Range |
31.25-2000pg/ml |
|
Alternative Names |
OL |
|
Detection Method |
Sandwich |
|
Sample Type |
Serum,plasma and other biological fluids |
|
Sample Volume |
100μL |
|
Detection Wavelength |
OD450 |
|
Transportation Temperature |
2-8°C |
|
Specificity |
The kit detected Human OxLDL in the samples and no significant cross-species reactions were found |
|
Microplate |
96-wells plate breakable into 12 x 8 wells strip |
|
Principle |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human OxLDL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OxLDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OxLDL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human OxLDL. You can calculate the concentration of Human OxLDL in the samples by comparing the OD of the samples to the standard curve. |
