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Human NFκB (Nuclear Factor Kappa B) Elisa Kit |
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Sensitivity |
0.188ng/ml |
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Range |
0.313-20ng/ml |
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Alternative Name |
NFKB ELISA Kit, Nuclear Factor Kappa B ELISA Kit, Nuclear factor NF-kappa-B p105 subunit ELISA Kit, DNA-binding factor KBF1 ELISA Kit, EBP-1 ELISA Kit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 ELISA Kit, NFKB1 ELISA Kit, Nuclear factor NF-kappa-B p100 subunit ELISA Kit, DNA-binding factor KBF2 ELISA Kit, H2TF1 ELISA Kit, Lymphocyte translocation chromosome 10 protein ELISA Kit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 ELISA Kit, Oncogene Lyt-10 ELISA Kit, Lyt10 ELISA Kit, NFKB2 ELISA Kit, LYT10 ELISA Kit |
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Sample Type |
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
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Detection Method |
Sandwich ELISA, Double Antibody |
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Wavelength |
OD450 |
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Reaction Duration |
4 hours |
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Storage |
2-8°C(Sealed), Don't cryopreserve. |
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Specificity |
Specifically binds with NFκB , no obvious cross reaction with other analogues. |
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Principle |
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti NFκB antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with NFκB bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of NFκB in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value. |
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Validated Image |
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