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Human Nuclear Factor-KappaB p65 (NF-κB p65) ELISA Kit |
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Sensitivity |
0.1μmol/L |
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Range |
1μmol/L -32μmol/L |
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Detection Method |
Sandwich |
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Test Principle |
Quantitative |
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Sample Type |
Serum, plasma, urine, saliva, stool, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
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Storage |
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to the manual |
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Test Principle |
The kit was used to test the level of Human Nuclear Factor-KappaB p65(NF-κB p65), based on the principle of double antibody sandwich technology enzyme linked immunosorbent assay (ELISA). Add Standard and Sample to the wells that pre-coated with objective antibody, then add HRP-Conjugate reagent to form an immune complex, incubation, by incubation and washing, removal of unbound enzyme, and then add the substrate A and B, then the solution will turn blue and finally change into yellow at the effect of acid. The color depth or light was positively correlated with the concentration of Nuclear Factor-KappaB p65 (NF-κB p65). |
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Human Nuclear Factor-KappaB p65 (NF-κB p65) ELISA Kit |
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Sensitivity |
0.1 ng/mL |
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Range |
0.16-10 ng/mL |
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Detection Method |
Sandwich |
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Alternative Names |
NFKB3, p65, RelA |
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Standard |
10 ng/mL |
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Sample Type |
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
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Assay Length |
3.5h |
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Test Principle |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Factor Kappa B p65(NFKB-p65). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Nuclear Factor Kappa B p65(NFKB-p65). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Factor Kappa B p65(NFKB-p65), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nuclear Factor Kappa B p65(NFKB-p65) in the samples is then determined by comparing the OD of the samples to the standard curve. |
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