| Uni Prot ID | Q16653 |
| Sample Type | Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
| Detection Method | Sandwich ELISA, Double Antibody |
| Detection Wavelength | OD450 |
| Reaction Duration | 4 hours |
| Storage | 2-8°C(Sealed), Don't cryopreserve |
| Specificity | Specifically binds with MOG , no obvious cross reaction with other analogues |
ELISA Kit Components
| Kit Components | Item | Size(48T) | Size(96T) | Storage Condition for Opened Kit |
| E001 | ELISA Microplate(Dismountable) | 8×6 | 8×12 | Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C |
| E002 | Lyophilized Standard | 1vial | 2vial | Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C |
| E003 | Lyophilized Biotin-labeled Antibody(Concentrated) | 1vial | 1vial | |
| E034 | HRP-Streptavidin Conjugate(SABC, 100X) | 60ul | 120ul | 2-8°C (Avoid Direct Light) |
| E024 | TMB Substrate | 5ml | 10ml | |
| E005 | Purified Water | 200ul | 200ul | 2-8°C |
| E039 | Sample Dilution Buffer | 10ml | 20ml | |
| E040 | Antibody Dilution Buffer | 5ml | 10ml | |
| E049 | SABC Dilution Buffer | 5ml | 10ml | |
| E026 | Stop Solution | 5ml | 10ml | |
| E038 | Wash Buffer(Concentrated, 25X) | 15ml | 30ml | |
| E006 | Plate Sealer | 3 pieces | 5 pieces | |
| E007 | Product Description | 1 copy | 1 copy |
Standard Curve
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.) Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
| STD.(ng/ml) | OD-1 | OD-2 | Average |
| 0 | 0.071 | 0.073 | 0.072 |
| 0.156 | 0.12 | 0.124 | 0.122 |
| 0.312 | 0.238 | 0.244 | 0.241 |
| 0.625 | 0.41 | 0.422 | 0.416 |
| 1.25 | 0.621 | 0.639 | 0.63 |
| 2.5 | 0.905 | 0.931 | 0.918 |
| 5 | 1.511 | 1.555 | 1.533 |
| 10 | 2.131 | 2.193 | 2.162 |
Recovery
Add a certain amount of MOG into the sample. Calculate the recovery by comparing the measured value with the expected amount of MOG in the sample.
| Sample Type | Recovery Range(%) | Average(%) |
| serum(n=10) | 88-104 | 96 |
| EDTA plasma(n=10) | 88-100 | 92 |
| Heparin plasma(n=10) | 86-103 | 95 |
Linearity
Dilute the sample with a certain amount of MOG at 1:2, 1:4 and 1:8 to get the recovery range.
| Sample Type | 1:2 | 1:4 | 1:8 |
| serum(n=10) | 89-98% | 90-105% | 86-105% |
| EDTA plasma(n=10) | 84-95% | 82-101% | 89-98% |
| Heparin plasma(n=10) | 87-97% | 85-97% | 84-99% |
Precision(%)
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate. Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
| Item | Intra-assay Precision | Inter-assay Precision | ||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/ml) | 0.31 | 1.26 | 5.07 | 0.34 | 1.2 | 5.18 |
| Standard deviation | 0.02 | 0.07 | 0.25 | 0.02 | 0.06 | 0.27 |
| CV(%) | 4.93 | 5.22 | 4.89 | 4.82 | 4.68 | 5.12 |
Stability
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
| ELISA kit(n=5) | 37°C for 1 month | 2-8°C for 6 months |
| Average(%) | 80 | 95-100 |
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