Description
| Sample | Serum | plasma | cell culture supernatant and other biological samples |
| For the quantitative determination of human Interleukin 6 (IL-6) concentrations in cell culture supernates, serum and plasma. | |
| PRINCIPLE OF THE ASSAY | This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for human IL-6 has been pre-coated onto a microplate. Standard, samples andbiotin-linked detect antibody specific for IL-6 are pipetted into the wells and IL-6 present is boundby the immobilized antibody and detect antibody following incubation. After washing away anyunbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
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| COMPONENTS | 96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-IL-6 monoclonal antibody
Human IL-6 freeze-dried standard IL-6 detect Antibody Standard Diluent Assay Buffer(10×) Substrate TMB Stop Solution Washing Buffer(20×) Sealing Film |
| Storage/Stability | 4℃ (unopened) for two years |
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Precautions |
The product listed herein is for research use only and not intended for diagnosis field.
The international literature is also constantly updated, so data sheet showed herein may not the latest reference data. It is best to confirm with us before ordering. The product listed herein all was produced by our laboratory. So, we can produce different system ELISA Kits. If you have any other requirements, please contact us, to get you wanted product information. |
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