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Human IL-10 (Interleukin 10) ELISA Kit - Details |
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Sensitivity |
0.28pg/mL |
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Range |
0.78-50pg/mL |
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Uniprot ID |
P22301 |
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Alternative Names |
Interleukin-10,IL-10,Cytokine synthesis inhibitory factor (CSIF),IL10,IL 10,CSIF |
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Detection Method |
Sandwich |
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Standard |
50pg/mL |
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Sample type |
Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL |
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Reaction time |
3.5H |
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Research Area |
Cancer, Immunology |
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Principle |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-10. You can calculate the concentration of Human IL-10 in the samples by comparing the OD of the samples to the standard curve. |
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Human IL-10 (Interleukin 10) ELISA Kit |
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Sensitivity |
0.3pg/ml |
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Range |
1.2pg/ml -100pg/ml |
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Detection Method |
Sandwich-ELISA |
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Standard |
135pg/ml |
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Sample type |
Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL |
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Sample Volume |
10ul |
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Wavelength |
450nm |
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Principle |
This ELISA kit uses Sandwich-ELISA as the method. The Micro Elisa strip plate provided in this kit has been pre-coated with an antibody specific to IL-10. Standards or samples are added to the appropriate Micro Elisa strip plate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for IL-10 is added to each Micro Elisa strip plate well and incubated. Free components are washed away. TheTMBsubstrate solution is added to each well. Only those wells that contain IL-10 and HRP conjugated IL-10 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at awave length of 450 nm. The OD value is proportional to the concentration of IL-10. You can calculate the concentration of IL-10 in the samples by comparing the OD of the samples to the standard curve. |
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Human IL-10 (Interleukin 10) ELISA Kit |
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Sensitivity |
3pg/mL |
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Range |
7.82-500pg/mL |
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Detection Method |
Sandwich |
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Alternative Names |
IL-10;CSIF; IL10A; TGIF; Cytokine Synthesis Inhibitory Factor |
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Sample type |
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
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Reaction time |
3.5h |
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Research Area |
Cytokine;Infection immunity |
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Principle |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 10(IL10). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 10(IL10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 10(IL10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 10(IL10) in the samples is then determined by comparing the OD of the samples to the standard curve. |
