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Human High Mobility Group Protein B1 (HMGB-1) ELISA Kit - Details |
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Sensitivity |
10pg/ml |
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Range |
1250pg/ml–40000pg/ml |
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Sample Type |
use with serum, plasma, culture media, or any biological fluids |
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Detection Method |
Quantitative Detection |
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Storage |
2-8℃ |
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Principle |
The quantitative detection of Human HMGB-1 antigen is based on the ELISA (Enzyme-Linked Immunosorbent Assay) technique. The microplate wells are pre-coated with an antibody specific to Human HMGB-1. After incubation, thecorresponding antigen in the sample or standards will bind to the immobilized antibody. After washing the microplate wells to remove all unbound sample material, horseradish peroxidase (HRP) labeled antibody conjugate is added, which binds to the antigen, forming antibody-antigen-enzyme-labeled antibody complex upon incubation. In a second wash step, all unbound material is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate, which gives a blue color reaction in wells containing the complex. A stop solution containing acid terminates the reaction, producing a yellow end-point coloration proportional to the amount of specific antigen present in the sample. Absorbance is measured at 450 nm using an ELISA microwell plate reader. Analysis is completed by comparing the OD values of samples to the standard curve. |
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Human High Mobility Group Protein B1 (HMGB-1) ELISA Kit |
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Sensitivity |
10.0pg/ml |
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Range |
100pg/ml -4000pg/ml |
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Sample Type |
use with serum, plasma, culture media, or any biological fluids |
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Detection Method |
Double Antibody Sandwich |
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Storage |
2-8℃ |
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Principle |
The kit was used to test the level of Human High Mobility Group Protein B1(HMGB-1), based on the principle of double antibody sandwich technology enzyme linked immunosorbent assay (ELISA).
Add Standard and Sample to the wells that pre-coated with objective antibody, then add HRP-Conjugate reagent to form an immune complex, incubation, by incubation and washing, removal of unbound enzyme, and then add the substrate A and B, then the solution will turn blue and finally change into yellow at the effect of acid. The color depth or light was positively correlated with the concentration of High Mobility Group Protein B1 (HMGB-1). |
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Human High Mobility Group Protein B1 (HMGB-1) ELISA Kit |
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Sensitivity |
10pg/mL |
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Range |
62.5-4000pg/mL |
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Sample Type |
use with serum, plasma, culture media, or any biological fluids |
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Detection Method |
Sandwich-ELISA |
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Storage |
2-8℃ |
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Principle |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HMGB-1. Samples (or Standards) are added to the micro ELISA plate wells
and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human HMGB-1. You can calculate the concentration of Human HMGB-1 in the samples by comparing the OD of the samples to the standard curve. |
