Product Name	Human HIF1A(Hypoxia-inducible factor 1-alpha) ELISA Kit
Synonyms	HIF-1α ELISA Kit, HIF1A ELISA Kit, ARNT-interacting protein ELISA Kit, Basic-helix-loop-helix-PAS protein MOP1 ELISA Kit, BHLHE78 ELISA Kit, Class E basic helix-loop-helix protein 78 ELISA Kit, HIF-1-alpha ELISA Kit, HIF1-alpha ELISA Kit, hypoxia-inducible factor 1-alpha ELISA Kit, Member of PAS protein 1 ELISA Kit, member of PAS superfamily 1 ELISA Kit, PAS domain-containing protein 8 ELISA Kit
Detection Method	Sandwich ELISA, Double Antibody
Application	HIF1A ELISA Kit allows for the in vitro quantitative determination of HIF1A concentrations in serum, plasma, tissue homogenates and other biological fluids.
Range	0.156-10ng/ml
Standard Curve
Storage	2-8 ℃ for 6 months
Recovery	Matrices listed below were spiked with certain level of HIF1A and the recovery rates were calculated by comparing the measured value to the expected amount of HIF1A in samples. Matrix Recovery range(%) Average(%) serum(n=5) 85-102 90 EDTA plasma(n=5) 89-100 96 heparin plasma(n=5) 93-105 98
Linearity	The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HIF1A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample 1:2 1:4 1:8 serum(n=5) 86-102% 89-104% 94-101% EDTA plasma(n=5) 82-93% 84-96% 88-100% heparin plasma(n=5) 86-96% 85-100% 88-99%
CV(%)	Intra-Assay: CV<8% Inter-Assay: CV<10%
Note	For Research Use Only

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