Purpose

Our Human hepatitis B virus e antigen (HBeAg) ELISA kit is for the qualitative determination of HBeAg in Human serum, plasma, culture media or any biological fluid.

Principle

The ELISA is based on the the qualitative enzyme immunoassay technique. The Microplate provided in this kit has been pre-coated with an Antibody specific to HBeAg, make it to solid-phase antibody. Samples are added to the Microplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)-conjugated antibody specific for HBeAg is added to each Microplate well and incubated ,so the antibody-antigen-Enzyme labeled antibody complex is formed. Following a wash to remove any unbound reagent, then the TMB substrate solution is added to each well. Only those wells that contain HBeAg and HRP conjugated HBeAg antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The qualitative determination of HBeAg is determined by comparing with the CUTOFF value.