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Human ENPP2 (Ectonucleotide pyrophosphatase/phosphodiesterase family member 2) ELISA Kit |
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Sensitivity |
0.75ng/ml |
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Range |
1.25-80ng/ml |
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Detection Method |
Sandwich ELISA, Double Antibody |
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Sample Type |
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples |
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Alternative Names |
Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 ELISA Kit, E-NPP 2 ELISA Kit, Autotaxin ELISA Kit, Extracellular lysophospholipase D ELISA Kit, LysoPLD ELISA Kit, ENPP2 ELISA Kit, ATX ELISA Kit, PDNP2 ELISA Kit |
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Assay length |
4 hours |
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Detection Wavelength |
OD450 |
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Uniprot ID |
Q13822 |
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Specificity |
Specifically binds with ENPP2 , no obvious cross reaction with other analogues. |
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Principle |
This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ENPP2 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with ENPP2 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of ENPP2 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value. |
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Validated Image |
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