|
Human Cor (Cortisol) Elisa Kit |
|
Sensitivity |
0.98 ng/mL |
|
Range |
3.13-200 ng/mL |
|
Detection Method |
Competitive Inhibition |
|
Sample Type |
serum, plasma, urine and other biological fluids |
|
Alternative Names |
Hydrocortisone |
|
Assay length |
2h |
|
Standard |
200 ng/mL |
|
Research Area |
Metabolic pathway;Endocrinology;Hormone metabolism |
|
Principle |
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Cor. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Cor. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Cor in the samples is then determined by comparing the OD of the samples to the standard curve. |
